Glutathione S-transferase (GST) (EC 184.108.40.206) is an enzyme that catalyzes the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates to detoxify endogenous compounds. The GST encoded by parasitic worm Schistosoma japonicum, which has little homology to its mammalian counterpart, had been widely used as a fusion patner for expression and purification of foreign proteins in E. coli. GST-tag is easy to detect and it has little effect on the biological function of the protein of interest. Alternatively, a GST-tag can be removed by enzymatic cleavage on particular site(s), which was expressed from a well-designed plasmid vector.
Our product is a mouse monoclonal antibody, which was raised against the full-length GST of S. japonicum origin. It is prepared as cell culture supernatant (~ 40 μg/ml) with 0.01% sodium azide. The GST (6F5) antibody detects GST-tag alone and its fusion proteins. It can be used for immunologic applications such as Western blotting (WB, dilution range: 1:50 - 500).
Gene Symbol (ID): GST (S. japonicum)
Molecular Weight: 26 kDa (GST)
Storage: Keep at 2 - 8°C upon arrival (stable for six months from the date of shipment). For long-term storage, make aliquots and keep them at -20°C or below. Avoid repeated freeze-and-thaw cycles.
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